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KMID : 0861020190340010023
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Korea Journal of Herbology
2019 Volume.34 No. 1 p.23 ~ p.31
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Effect of Fritillariae Thunbergii Bulbus for Allergic Inflammationon HaCaT and RBL2H3 Cells
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Kim Eun-Young
Lee Bi-Na
Kim Jae-Hyun
Hong Soo-Yeon
Kim Min-Seon
Park Jae-Ho
Kim Jwa-Jin
Sohn Young-Joo
Jung Hyuk-Sang
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Abstract
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Objectives : The aim of this study was to investigate the effect for allergic-inflammation of Fritillariae Thunbergii Bulbus (FTB) on HaCaT cells and RBL2H3 cells.
Methods : To investigate the effects of FTB for anti-inflammation in HaCaT cells, the cells were pretreated with FTB for 1h and then stimulated with TNF-/IFN- for 24h. Then thymus and activation-regulated chemokine (TARC) and Macrophage-derived chemokine (MDC) levels were analyzed with ELISA kit.
Also to investigate the effect of skin barrier protein, the cells were treated with FTB of various concentrations, and then cells were harvested, expressions of skin barrier protein were measured with RT-PCR. To investigate the effects of FTB for anti-allergy in RBL2H3 cells, the cells were pre-treated with FTB for 1h, and then stimulated with A23187 for 30 min. -hexosaminidase, IL-4 and TNF- were measured using cultured media. The cells were harvested to analyze the mechanism of the effect for FTB via Western blot.
Results : FTB did not show cytotoxicity in HaCaT and RBL2H3. In HaCaT cells, FTB significantly suppressed the expression of TARC, MDC at a dose-dependent manner and markedly increased formation of the skin barrier proteins. In RBL2H3 cells, FTB decreased release of the -hexosaminidase, IL-4 and TNF- in RBL2H3 through inhibition of the phosphorylation of JNK and p38, which are include in the signaling mechanism of MAPK
Conclusion : These results indicate that FTB has an anti-inflammatory effect on the allergic response through blocking MAPK pathway. This suggest that FTB could be a therapeutic agent for allergic response.
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KEYWORD
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Fritillariae Thunbergii Bulbus, Allergy, HaCaT, RBL2H3
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